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Aberrant Cas9 expression from mammalian promoters in E. coli causes guide-specific toxicity that skews CRISPR library representation in all-in-one Cas9 vectors. Replacing EF-1α with PGK reduces ...

Much of what shapes people’s lives happens at an unseen level—it comes down to DNA. This year, The Scientist explored genetic insights into how individuals smell, metabolize alcohol, and even how ...

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